Team:Paris-Saclay/Project/Notebook/Week 4

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Week 4

  • We placed an order for the primers.
  • DNA (biobricks) Resuspension using iGEM protocol.
  • Thermal shock treatment of each biobrick to have a large quantity of DNA.
  • Staggering of the transformed bacteria on a selective medium to select bacteria which recieved the plasmid.
  • We started some colonies, one per biobrick, on a liquid culture, in order to make a Miniprep.
  • Miniprep to purify each biobrick (ready to use).
  • Groove for two colonies because they did not grow on the liquid culture.